phenol and biotinylated DNA

Matt Thomas mthomas at hsc.usf.edu
Wed Feb 7 15:10:19 EST 2001


I'm wondering does doing a  phenol extract and Etol ppt of DNA that has
been biotinyloated or an oligo with biotin at the 5' end mess things
up?  Basically, can I label some DNA this way clean it up and still have
it bind to strepavadin ok?

thanks
Matt thomas
___________________________________________
Matthew Thomas, Ph. D
H. Lee Moffitt Cancer Center, University of South Florida
mthomas at hsc.usf.edu

"I refrain from publishing for fear that disputes and
controversies may be raised against me by ignoramuses."
   Sir Isaac Newton, correspondence to Liebniz






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