PWO PCR after T4 Ligation

Susanne Rohrer srohrer.minus at this.immv.unizh.ch
Mon Feb 12 06:16:59 EST 2001



Christof Gaenzler wrote:

> Which buffer do I need for a PWO PCR directly after a T4 Ligase reaction?
>

I don't know what you want to do (inverse PCR maybe) but I recommend you
dilute your Ligation anyway (say use only 1ul per 50ul PCR) and use normal
Pwo buffer with Mg.

Susanne






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