PWO PCR after T4 Ligation

Peter Ashby p.r.ashby at
Mon Feb 12 08:00:54 EST 2001

In article <3A87C621.3C40C23 at>, 
nospam at wrote:

> Christof Gaenzler wrote:
> > Which buffer do I need for a PWO PCR directly after a T4 Ligase 
> > reaction?
> >
> I don't know what you want to do (inverse PCR maybe) but I recommend you
> dilute your Ligation anyway (say use only 1ul per 50ul PCR) and use 
> normal
> Pwo buffer with Mg.

I've done exactly what Suzanne suggests with ordinary Taq and it worked 
very well.


Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
Reverse the spam and remove to email me.

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