PWO PCR after T4 Ligation

Peter Ashby p.r.ashby at dundee.MAPS.ac.uk
Mon Feb 12 08:00:54 EST 2001


In article <3A87C621.3C40C23 at this.immv.unizh.ch>, 
nospam at rzu-mailhost.unizh.ch wrote:

> Christof Gaenzler wrote:
> 
> > Which buffer do I need for a PWO PCR directly after a T4 Ligase 
> > reaction?
> >
> 
> I don't know what you want to do (inverse PCR maybe) but I recommend you
> dilute your Ligation anyway (say use only 1ul per 50ul PCR) and use 
> normal
> Pwo buffer with Mg.

I've done exactly what Suzanne suggests with ordinary Taq and it worked 
very well.

Peter

-- 
Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
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