Remove DNA from protein prep

Roland Saldanha rsaldanh at mail.utexas.edu
Wed Feb 14 19:06:10 EST 2001


In article <3FA493B792A6D311B51A009027D3B861245007 at mail.kgi.edu>,
Bernard_Delacruz at kgi.edu (Bernard Delacruz) wrote:

> I want to use a crude protein prep to do
> a gel-shift assay but I seem to have DNA
> in the prep. Does anyone have a suggestion
> for removing the DNA? I thought about using
> DNAse, but then how do I get the DNAse
> out?
> 
> Thanks.


I have used Micrococcal nuclease - it is Calcium dependant and you can
chelate out the calcium with EGTA.  

Roland


> 
> 
> ---






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