Separation of 15kD proteins by SDS-PAGE

Paul S. Brookes. brookes at uab.edu
Thu Feb 15 09:33:45 EST 2001


For various reasons, Tris-Tricine gels are far superior to Tris-Glycine 
(Laemmli) Gels for low molecular weight proteins and peptides.  See 
<Schagger H & von Jagow G (1987) Anal. Biochem. vol 166, p368-379> for a 
comprehensive method.

Regards
PSB

_________________________________________
Dr. Paul S. Brookes.            (brookes at uab.edu)
UAB Department of Pathology,   G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915     Fax (001) 205 934 1775
http://peir.path.uab.edu/brookes

The quality of e-mails can go down as well as up
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