primers problem

Wolfgang Schechinger marsupilamiwolfgang at pop3.web.de
Fri Feb 16 00:29:14 EST 2001


EDTA could affect those enzymes liking bivalent cations, like Mg2+.
As well, you introduce a buffering substance (tris) into the system.
For me, water does the job pretty well.

All the best,

Wolfgang

> Hi Tina,
>
> I always resuspend oligos at about 100 µM in TE (pH 8.0) then aliqout and
> store at -20°C.
>
> Tim
>
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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica
Taipei 115
Taiwan R.O.C.


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