Fugene conditions

Bruno Cenni bruno.removethis.cenni at removethis.insel.ch
Fri Feb 16 08:59:44 EST 2001

Hi all,

we're trying to use Fugene6 on MG-63 cells. From what's in the manual, it's
not clear what should be the volume of medium used to initially dilute the
fugene in. In their example for the 35 mm dishes they say 100 ul. We tried
scaling it down to 25 ul or 50 ul for for 24 wells (proportionally to the
scaled-down fugen/DNA amounts per well) and that seemed pretty bad.

Any comments?


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