Separation of 15kD proteins by SDS-PAGE

Bernard Delacruz Bernard_Delacruz at
Fri Feb 16 19:06:10 EST 2001

I'm not really a protein person but this came up
in a lab I was working in that they were losing
a ~20 kD protein they were working on because it
was going *through* the membrane during transfer.
It may seem obvious, but it took a few weeks before
they realized it loss of the protein and not
detection was the problem.

Good luck.


More information about the Methods mailing list