Ribozyme vectors

Peter Ashby p.r.ashby at dundee.MAPS.ac.uk
Mon Feb 19 05:42:54 EST 2001


In article <sa91077e.088 at baker.edu.au>, Zhonglin.Chai at baker.edu.au 
("Zhonglin Chai") wrote:

> Hello Everyone,
> 
> I am looking for a mammalian expressing vector containing a hammerhead 
> ribozyme core sequence and convenient cloning sites where I can put the 
> gene specific oligos in. I need this kind of vector to make constructs to 
> express gene specific ribozymes. All the comments, suggestions and 
> information are mostly welcome.

This one is chicken but it may work in mammals, check out Zhao and 
Lemke, 1998 Development 125; 1899-1907 and the accompanying methods 
paper: Zhao and Lemke 1998 Mol. Cell. Neurosci.  Sorry, can't find my 
copy for further details.

Peter

-- 
Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
Reverse the spam and remove to email me.






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