Extraction from Agarose-gels

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Feb 21 04:58:18 EST 2001

When I was making probes for Southerns I just used a freeze squeeze
method.  How much probe DNA do you need though?  Mike.

On Tue, 20 Feb 2001, Maik wrote:

> Hey folks,
> recently I am working on radioactive probes. I have to extract my target
> DNA for the probe from an agarose gel before. I have used Quiagen
> Extraction Kit so far, but I am loosing a lot of Target-DNA this way.
> Does anybody know a better kit or method to get clean enough and
> sufficient DNA for making radioactive probes?

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