Shearing of DNA

David F. Spencer dspencer at is.dal.ca
Wed Feb 21 17:30:38 EST 2001


In article <3A8C4E44.7881871E at mail.ncifcrf.gov>, ddaelemans at ncifcrf.gov 
(Dirk Daelemans) wrote:

> I would like to shear DNA to fragments of about 300-500 bp. Can anyone
> recommend an easy method that gives a nice distribution (not bigger than
> 1000 not smaller than 200 bp) of sheared DNA?
> 
> Thanks
> 
> 
> ---

Two passes through a French pressure cell at 16,000 psi will give a 
fairly tight size distribution centered at about 600 bp.

Dave

-- 
David F. Spencer, PhD
Dept. of Biochemistry and Molecular Biology
Dalhousie University
Halifax, Nova Scotia, Canada

dspencer at is.dal.ca






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