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Harjeet harjeet at sgpgi.ac.in
Thu Feb 22 14:05:22 EST 2001

Hello! Everybody
I  am trying to measure the enzymatic activity of Activated MAPkinae
(P44) by immunoprecipitating theMAPK from  cytosolic fraction of LPS
stimulated as well as non stimulated mouse MAcrophage cells ( RAW
264.7). I Have used anti MAPKinase Antibody (SIGMA)against
phosphorylated form.
But the problem is that I am getting less icorporation of P32 in
substrate(MBP) in lps treated group in comparison to unstimulated cells.

kindly help me please.

prem (premn at sgpgi.ac.in)

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