harjeet at sgpgi.ac.in
Thu Feb 22 14:05:22 EST 2001
I am trying to measure the enzymatic activity of Activated MAPkinae
(P44) by immunoprecipitating theMAPK from cytosolic fraction of LPS
stimulated as well as non stimulated mouse MAcrophage cells ( RAW
264.7). I Have used anti MAPKinase Antibody (SIGMA)against
But the problem is that I am getting less icorporation of P32 in
substrate(MBP) in lps treated group in comparison to unstimulated cells.
kindly help me please.
prem (premn at sgpgi.ac.in)
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