Searching a genome to find primer sequences that do not occur

Warren Gallin wgallin at gpu.srv.ualberta.ca
Mon Feb 26 11:44:32 EST 2001


Duncan,
	Most sequences will not be there (4 to the 20th power possible
sequences vs. the length of the actual genome you are looking at), so I
don't understand what you are after.  Do you mean take a list of
sequences and see which ones are not in the genome?

Warren Gallin

"Dr. Duncan Clark" wrote:
> 
> Hi Folks,
> 
> Is there any way to search a small genome to find primer sequences that
> do not exist in that genome e.g. say anything from 15-22mers.
> 
> I know one can search for any particular sequence but this is kind of a
> reverse search, looking for something that isn't there.
> 
> Any pointers?
> 
> Many thanks
> 
> Duncan
> 
> 
> --
> The problem with being on the cutting edge is that you occasionally get
> sliced from time to time....
> 
> Duncan Clark
> GeneSys Ltd.
> Tel: +44(0)1252376288
> FAX: +44(0)8701640382
> http://www.genesys.demon.co.uk
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