Urea assay

Arnoud van Vliet avvliet at knoware.nl
Mon Feb 26 19:23:26 EST 2001


>
> Does anybody know a method to determine the concentration of Urea in an
> aqueous solution ?

Claus,
Sigma sells a kit to do it. It uses urease, which degrades urea to ammonia
and CO2. The ammonia is then detected using a color reaction. Using
standards one can then easily determine how much urea is in your solution.
Check www.sigma-aldrich.com, Sigma Diagnostics, product 640. No
affiliations, just a satisfied user. Used it to determine urease
activity in bacterial lysates, actually)

hope this helps
Arnoud










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