Sonication of DNA for cloning

salanti ali at image.dk
Tue Feb 27 04:42:12 EST 2001


I am going to make a small library from a 7kb PCR fragment. I plan to
fragment the big PCR product by sonication, then treat it with Klenow.
But what happens to the "nicks" in the DNA that will occur from the
sonication?
In a protocol using DNAse 1 to digest the DNA, they point out that it is
important to use a Mn+ buffer instead of Mg+ in order to avoid nicking of
DNA!! My question is : Is it OK to sonicate DNA for cloning purposes

Thank you
Salanti







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