RNA bands

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Feb 27 06:25:17 EST 2001


In article <3A9B8641.C6FD5A1E at ipb.uni-halle-de>, the eminent Sudha Sahay
at Leibniz-Institut für Pflanzenbiochemie wrote
>DNAse can be removed by
>phenol/chloroform and by using ethanol, you can easily precipitise and
>clean your RNA. This is the only way you can use.

DNase can also be killed by pepsin at low pH and then the pepsin killed
by shifting buffer back to around pH8.

Published in Analytical Biochemistry maybe six months or so ago. One of
the quick reports at the back. Haven't tried it but it looked a good
idea. 

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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