rapid ligation buffer
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Tue Feb 27 10:55:27 EST 2001
I personally like the PEG-containing buffer formulation that Gibco
suggests. The formulation is in their catalog. I find that for routine
(sticky ends with normal sized vectors and inserts), 15-30 min incubation
is often sufficient. In my opinion, usually ligation times are not the
limiting factor in a cloning experiment, growing up the transformants is!
>Does anybody know how to prepare this rapid ligation buffer which is
>sold quite expensive in rapid ligation kits, but in fact works with any
>T4 DNA ligase?
More information about the Methods