Sonication of DNA for cloning

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue Feb 27 20:37:57 EST 2001


Why a library from such a small fragment?

Whatever the end use, I'm sure there are better ways to arrive there.
Sonicating a 7kb DNA may not be a very fruitful idea.


At 10:42 AM 2/27/01 +0100, salanti wrote:
>I am going to make a small library from a 7kb PCR fragment. I plan to
>fragment the big PCR product by sonication, then treat it with Klenow.
>But what happens to the "nicks" in the DNA that will occur from the
>sonication?
>In a protocol using DNAse 1 to digest the DNA, they point out that it is
>important to use a Mn+ buffer instead of Mg+ in order to avoid nicking of
>DNA!! My question is : Is it OK to sonicate DNA for cloning purposes
>
>Thank you
>Salanti
>
>
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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