pI/mW of multiple sequences

Mitchell Isaacs misaacs at spam_me_not.student.usyd.edu.au
Mon Jan 1 01:37:09 EST 2001


"Michael Witty" <mw132 at mole.bio.cam.ac.uk> wrote in message
news:Pine.SGI.3.96.1001231220504.14947613B-100000 at mole.bio.cam.ac.uk...
> Do we thing that theoretical pI's are good estimates?  Is there a review
> of theoretical versus experimentally determined pI's?  Mike
I don't know of any, but while the observed & theoretical pI's can vary
rather enormously, it does give a good general idea. From my experience (not
huge I'll admit) the majority of observed pI's fall within <1 unit of the
theoretical pI.

If you're looking at a broad range (eg 4-7, 3-10) it can be quite helpful to
know the theoretical pI.

Cheers,
Mitchell







More information about the Methods mailing list