[Q] How to extract the golgi apparatus

engelbert_buxbaum4 at my-deja.com engelbert_buxbaum4 at my-deja.com
Mon Jan 1 08:59:06 EST 2001


In article <92lobh$ut3$05$1 at news.t-online.com>,
  "Ulrik" <Ulriks at ruc.dk> wrote:
>
> Is it possible to extract the golgi apparatus, and how?


What you have to do is to gently homogenise your tissue (breaking the
cells but not the organelles) and then separate the various organelles
by a series of centrifugation steps. A useful source for detailed info
on this (and many other things) is the 'Methods in Enzymology' series,
which you will find in any well stocked library.

This should be enough of a pointer to get you started on your project.


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