Why wet blot cause non-specific signal

Kittapong Tang moota at hotmail.com
Tue Jan 2 11:50:50 EST 2001

Hi All,
   I have some problems in protein botting. Why wet blot give more signal 
than semi-dry blot even with the same sample and the same blotting 
efficiency? Can wet blot change the protein conformation?

Thanks in advance for all help.

Tang K.
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