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protein reduction

Sophie Le Page slepage at umich.edu
Thu Jan 4 15:18:59 EST 2001

Does anybody know what concentration of reducing agent (DTT or
mercaptoethanol) would be required in order to completely reduce a
protein containing 8 sulfhydryl groups? So far I've tried lysis buffer
containing 10mM DTT combined with 50mM mercaptoethanol but the protein
still doesn't reduce completely.



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