appearance of "clean" DNA?

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Fri Jan 5 04:58:58 EST 2001


Hi
I have no idea about plants, but my bacterial genomic DNA is quite viscous
when I get a high yield.

Susanne

mwcrepeau at my-deja.com wrote:

> What does clean genomic DNA look like?  It should, of course, form a
> clear, colorless solution when dissolved in TE buffer, but what about
> viscosity?  I remember a graduate student looking at one of my first
> plant DNA preparations when I was un undergrad and telling me that
> there was a good yield because the prep was very viscous.
>
> Later, someone else told me that DNA itself is not viscous in solution
> and that any viscosity is due to contaminants.  That seems reasonable,
> but it has also been my experience that the viscosity is almost
> impossible to eliminate, even with several additional phenol/chloroform
> extractions and/or ethanol precipitations.
>
> What is responsible for the viscosity that is typical of plant genomic
> DNA preps?  Moreover, if I could get my genomic DNA completely clean,
> so that nothing was left but the nucleic acid itself, what would it
> look like in solution?  And what would it look like as an ethanol
> precipitate?  Would it appear as the white cloud that I am accustomed
> to seeing, or is the white color due to bound protein and/or other
> contaminants?
>
> Perhaps this is just a theoretical question, since it may not be
> possible to obtain completely clean genomic DNA, but I'm still curious
> what people think about it.
>
> Marc Crepeau
> <mwcrepeau at hotmail.com>
>
> Sent via Deja.com
> http://www.deja.com/






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