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Secondary Ab in Westerns

Robert Hartley rh at mblab.gla.ac.uk
Fri Jan 5 20:07:15 EST 2001

In article <3A55C112.9AC56A96 at nki.nl>, dkram at nki.nl (duco kramer) wrote:

> Dear reader
> I am looking for a comparision of peroxidase conjugated secondary
> antibodies (anti mouse and anti rabbit) to be used with
> chemiluminescence on western blots.

VECTOR labs make good 2nd Ab but you might find that that the best bet is
to use good ECL reagents like the Amersham ones.

If you think about it haveing less efficient Ab's will have the same lack
of eficiency on both controml and test.

I personally use the National diagnostic ones that are free in Scotland.
Ahhh the good old NHS :-)

Bob; Sunny Scotland
Robert Hartley, Centre for Cell Engineering,University of Glasgow,
Glasgow G12 8QQ Tel:++44(0)141 330 4756
Fax:++44 (0)141 330 3730 mailto:rh at mblab.gla.ac.uk
Web : http://www.gla.ac.uk/Inter/CellEngineering

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