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FPLC and crumbling rubber o-rings

John Philo "jphilo*NO SPAM12*" at earthlink.net
Fri Jan 5 19:18:12 EST 2001

I can't offer any specific advice about alternative o-rings, but since
I've not encountered or heard of this problem with FPLCs, I'm suspicious
that there is something in your lab environment that is causing this. In
my experience, ozone is usually the culprit in labs where rubber objects
die rapidly. Is there a UV lamp near your FPLC (other than the one in
your absorbance monitor for the FPLC, which I don't think causes such

John Philo
Alliance Protein Laboratories

"T. Max M. Soegaard" wrote:
> Hi all.
> So, we have this FPLC-apparatus from Pharmacia..
> Pharmacia service costs are high, so we usually service it ourselves.
> Problem is the rubber o-rings in the pumps. They crumble and leave
> rubber-dust that clogs up the filters.
> Tried different types of rubber - no luck - even the Pharmacia ones
> crumble.
> Have anyone out there found a supplier of O-rings that are able to
> successfully endure both the chemical and the physical stress of being
> shoved around in a glass shaft for months?
> Any help appreciated greatly.
> - Max

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