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Secondary Ab in Westerns

Stephen Snowdy snowds at med.unc.edu
Sat Jan 6 15:24:04 EST 2001

I've also found that the ECL reagents are extremely important.
We use the WestPico substrate from Pierce and usually get more
sensitivity than we really want.  We get good results with Amersham
secondary HRP, but haven't really compared it to other brands.
Best of luck.

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