FPLC and crumbling rubber o-rings

soren k rasmussen soren.rasmussen at risoe.dk
Sun Jan 7 11:14:52 EST 2001


we have similar problems with O-rings, could be caused by 8 M Urea?

--
soren.rasmussen at risoe.dk
John Philo <"jphilo*NO SPAM12*"@earthlink.net> > wrote in message
<3A56644B.A33B22C2 at mailway.com>...
>I can't offer any specific advice about alternative o-rings, but since
>I've not encountered or heard of this problem with FPLCs, I'm suspicious
>that there is something in your lab environment that is causing this. In
>my experience, ozone is usually the culprit in labs where rubber objects
>die rapidly. Is there a UV lamp near your FPLC (other than the one in
>your absorbance monitor for the FPLC, which I don't think causes such
>problems)?
>
>John Philo
>Alliance Protein Laboratories
>
>"T. Max M. Soegaard" wrote:
>>
>> Hi all.
>> So, we have this FPLC-apparatus from Pharmacia..
>> Pharmacia service costs are high, so we usually service it ourselves.
>> Problem is the rubber o-rings in the pumps. They crumble and leave
>> rubber-dust that clogs up the filters.
>> Tried different types of rubber - no luck - even the Pharmacia ones
>> crumble.
>>
>> Have anyone out there found a supplier of O-rings that are able to
>> successfully endure both the chemical and the physical stress of being
>> shoved around in a glass shaft for months?
>>
>> Any help appreciated greatly.
>> - Max







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