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two-hybrid suggestions

Burkhard Hassel Burkhard.Hassel at t-online.de
Mon Jan 8 18:21:57 EST 2001

On 7 Jan 2001 20:17:28 -0000, rondi.butler at umit.maine.edu (Rebecca Van
Beneden) wrote:

>Our lab is preparing to venture into the world of protein-protein
>interactions/yeast two-hybrids.  I am looking for any
>suggestions/personal experience anyone might have with commercially
>available kits or procedures from 'do-it-yourself'-ers.  Any helpful
>suggested readings would also be rgeatly appreciated.  Perhaps the
>greatest complaint I have heard (in my limited exposure thus far) is
>library construction.  Are there companies that will make libraries?
>(in case we win lotto) We work with a non-traditional organism (clam) so
>no pre-transformed libraries are available :(
>Thanks in advance for your help.
>R. Butler

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