two-hybrid suggestions

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Tue Jan 9 04:47:49 EST 2001


"Burkhard Hassel" <Burkhard.Hassel at t-online.de> schrieb im Newsbeitrag
news:3a5a4a88.1592984 at news.btx.dtag.de...
> On 7 Jan 2001 20:17:28 -0000, rondi.butler at umit.maine.edu (Rebecca Van
> Beneden) wrote:
>
> >Our lab is preparing to venture into the world of protein-protein
> >interactions/yeast two-hybrids.  I am looking for any
> >suggestions/personal experience anyone might have with commercially
> >available kits or procedures from 'do-it-yourself'-ers.  Any helpful
> >suggested readings would also be rgeatly appreciated.  Perhaps the
> >greatest complaint I have heard (in my limited exposure thus far) is
> >library construction.  Are there companies that will make libraries?
> >(in case we win lotto) We work with a non-traditional organism (clam) so
> >no pre-transformed libraries are available :(
> >
> >Thanks in advance for your help.
> >
> >R. Butler
I recommend the Two Hybrid System 3 from Clontech (no affil.); I
successfully used it with a pretransformed library; it is supplied with
detailed instructions and it worked well in my hands.
Martin







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