IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

What is the effect of a 5' overhang on gel mobility?

WILLIS GAVIN RM1Norfolk and Norwich NHS Trust gavin.willis at norfolk-norwich.thenhs.com
Tue Jan 9 09:48:29 EST 2001

I want to make a (blunt ended) PCR product that will migrate at the same
speed as a restriction fragment with one blunt end and a 4 b.p. 5' overhang.
Should the blunt PCR product be as long as the fragment including the length
of the overhang, not including it or in between?

Gavin Willis

The information contained in this e-mail is confidential and is intended
only for the named recipient(s). If you are not the intended recipient you
must not copy, distribute, or take any action or reliance on it. If you have
received this e-mail in error, please notify the sender. Any unauthorised
disclosure of the information contained in this e-mail is strictly


More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net