What is the effect of a 5' overhang on gel mobility?

WILLIS GAVIN RM1Norfolk and Norwich NHS Trust gavin.willis at norfolk-norwich.thenhs.com
Tue Jan 9 09:48:29 EST 2001


I want to make a (blunt ended) PCR product that will migrate at the same
speed as a restriction fragment with one blunt end and a 4 b.p. 5' overhang.
Should the blunt PCR product be as long as the fragment including the length
of the overhang, not including it or in between?

Thanks
Gavin Willis


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