Fw: What is the effect of a 5' overhang on gel mobility?

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Wed Jan 10 21:31:54 EST 2001


Do you really know what you are talking about?
>
>
>I haven't really done any of this work but i would think that 4 bp would
>make a difference.
>When you run restriction fragments out on a gel, I think the fragments are
>denatured and run in such a manner that you have resolution to 1 - 2 bp.
>Kind of like a sequencing gel.  In my humble opinion (IMHO) if you want
>these two fragments to migrate down a gel at the same speed, you should
>make the PCR product as long as the restriction fragment including the
>overhang.  Then denature all the fragments and run the gel.
>
>Deanne Bell
>Molecular Markers Lab Technician
>USDA Agricultural Research Service
>2021 S. Peach Ave.
>Fresno, CA 93727
>e-mail: dbell at qnis.net
>phone: 559 453-3170
>fax: 559 453-3088
>
>
>---
>
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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