Do you really know what you are talking about?
>>>I haven't really done any of this work but i would think that 4 bp would
>make a difference.
>When you run restriction fragments out on a gel, I think the fragments are
>denatured and run in such a manner that you have resolution to 1 - 2 bp.
>Kind of like a sequencing gel. In my humble opinion (IMHO) if you want
>these two fragments to migrate down a gel at the same speed, you should
>make the PCR product as long as the restriction fragment including the
>overhang. Then denature all the fragments and run the gel.
>Molecular Markers Lab Technician
>USDA Agricultural Research Service
>2021 S. Peach Ave.
>Fresno, CA 93727
>e-mail: dbell at qnis.net>phone: 559 453-3170
>fax: 559 453-3088
>>>Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Dept. of Chemistry & Biochemistry
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003
Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu