Small scale TRIZOL extraction

Susanne Rohrer "srohrer(removethis)" at
Thu Jan 11 04:31:33 EST 2001

Aki Hoji wrote:

> Dear all,
> I'd very much appreciate if somebody lets me know a recommended volume of
> TRIZOL for small size samples (10-20ul).  Thanks in advance.

I once isolated RNA from a couple day 3 chicken embryos in about 500ul
Trizol. (for RT-PCR)
Maybe that helps.


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