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what is the effect of a 5' overhang on gel mobility

WILLIS GAVIN RM1Norfolk and Norwich NHS Trust gavin.willis at norfolk-norwich.thenhs.com
Thu Jan 11 07:15:29 EST 2001

The product will be about 25bp. The reason I want it to migrate at the same
speed is that there is another 25bp fragment that will be present in mutant
individuals. I will then get 50 bp =normal, 25 bp = mutant. Because thy are
so small the fragments are pretty faint so co migration would be nice though
not essential. Under the conditions I am using there is also a nice window
around 25 bp between  primer related artefact and other restriction
fragments. I don't want to denature because that would make the fragments
hard to see and the pattern more complex.


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