According to your question, did you used the same type of membrane
for comparing the result between wet-blot and semi-dry blot?
Also, how did you confirm that both system have the equal
transfer efficiency. I usually found that wet blotting is
more efficient in transfering protein from acrylamide gel
than the semi-dry blotting.
I think, your case might be caused by different transfer efficiency
between both methods.
jongrak at hotmail.com
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.