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Small scale TRIZOL extraction

Bill Blackhall b.blackhall at staff.usyd.edu.au
Thu Jan 11 16:53:32 EST 2001

Aki Hoji wrote:

> Dear all,
> I'd very much appreciate if somebody lets me know a recommended volume of
> TRIZOL for small size samples (10-20ul).  Thanks in advance.
> Aki Hoji
> University of Pittsburgh
> Department of Infectious Diseases and Microbiology
> Rm522 Parran Hall, GSPH-IDM
> 130 Desoto Street, Pittsburgh PA 15261
> tel: (412) 624-3072/0776
> fax: (412) 624-4953

I've extracted both RNA and DNA from the same samples of individual nematodes
(~ 1 cm in length) in 200 ul Trizol. Got enough RNA from each worm to do
about 100 RT-PCRs.

Bill Blackhall
University of Sydney

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