Aki Hoji wrote:
> Dear all,
>> I'd very much appreciate if somebody lets me know a recommended volume of
> TRIZOL for small size samples (10-20ul). Thanks in advance.
>> Aki Hoji
> University of Pittsburgh
> Department of Infectious Diseases and Microbiology
> Rm522 Parran Hall, GSPH-IDM
> 130 Desoto Street, Pittsburgh PA 15261
> tel: (412) 624-3072/0776
> fax: (412) 624-4953
I've extracted both RNA and DNA from the same samples of individual nematodes
(~ 1 cm in length) in 200 ul Trizol. Got enough RNA from each worm to do
about 100 RT-PCRs.
University of Sydney