Blunting sheared DNA

Chris Upton cupton at uvic.ca
Fri Jan 12 18:14:12 EST 2001


Hi, we're going to be generating randomly sheared DNA for sequencing using
a nebulizer, and cloning into an Invitrogen Topo-Blunt vector.

Anyone have any suggestions for blunting the ends?
We have some of the Stratagene  PCR polishing kit around that uses Pfu.
Anyone tried this to fix sheared DNA?

Thanks,
  Chris
-- 
Dept Biochemistry and Microbiology
University of Victoria
Victoria BC






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