Never done with sheared DNA, but I would think that T4 DNA polymerse would
work with its 3-5' exo and 5-3' poly activity.
At 03:14 PM 1/12/01 -0800, Chris Upton wrote:
>Hi, we're going to be generating randomly sheared DNA for sequencing using
>a nebulizer, and cloning into an Invitrogen Topo-Blunt vector.
>>Anyone have any suggestions for blunting the ends?
>We have some of the Stratagene PCR polishing kit around that uses Pfu.
>Anyone tried this to fix sheared DNA?
>Dept Biochemistry and Microbiology
>University of Victoria
>>>Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Dept. of Chemistry & Biochemistry
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003
Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu