Blunting sheared DNA

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Fri Jan 12 21:06:51 EST 2001


Never done with sheared DNA, but I would think that T4 DNA polymerse would
work with its 3-5' exo and 5-3' poly activity.

At 03:14 PM 1/12/01 -0800, Chris Upton wrote:
>Hi, we're going to be generating randomly sheared DNA for sequencing using
>a nebulizer, and cloning into an Invitrogen Topo-Blunt vector.
>
>Anyone have any suggestions for blunting the ends?
>We have some of the Stratagene  PCR polishing kit around that uses Pfu.
>Anyone tried this to fix sheared DNA?
>
>Thanks,
>  Chris
>-- 
>Dept Biochemistry and Microbiology
>University of Victoria
>Victoria BC
>
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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