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cloning PCR products

Yin Wang yin.wang at weizmann.ac.il
Sun Jan 14 11:16:10 EST 2001

Hi all,
I'm looking for an efficient way to clone 1.6 and 2 kb PCR products into
pET 20b(+) vector,after trying in vain with cohesive end[EcoR1 ,Hind111]
ligation for months.
Thanks in advance!!

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