pI/mW of multiple sequences

Paul O'Donnell pao at unimelb.edu.au
Sun Jan 14 18:46:23 EST 2001


This site has pI calculator and many other protein tools
http://jpsl.ludwig.edu.au/

Mitchell Isaacs wrote:

> "Michael Witty" <mw132 at mole.bio.cam.ac.uk> wrote in message
> news:Pine.SGI.3.96.1001231220504.14947613B-100000 at mole.bio.cam.ac.uk...
> > Do we thing that theoretical pI's are good estimates?  Is there a review
> > of theoretical versus experimentally determined pI's?  Mike
> I don't know of any, but while the observed & theoretical pI's can vary
> rather enormously, it does give a good general idea. From my experience (not
> huge I'll admit) the majority of observed pI's fall within <1 unit of the
> theoretical pI.
>
> If you're looking at a broad range (eg 4-7, 3-10) it can be quite helpful to
> know the theoretical pI.
>
> Cheers,
> Mitchell






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