cloning PCR products

Robert Hartley rh at
Mon Jan 15 11:53:05 EST 2001

In article <3A61D0C9.5C3442D0 at>, at wrote:

For a strategy plan have a look at Gene Cloning by RT-PCR. Siebert and
Larick, specifically the chapter by Steve Winder.

This will show a comparison of vector/method etc (not specific to your
vector but worth a read.

Bob; VERY Sunny Scotland
> Hi all,
> I'm looking for an efficient way to clone 1.6 and 2 kb PCR products into
> pET 20b(+) vector,after trying in vain with cohesive end[EcoR1 ,Hind111]
> ligation for months.
> Thanks in advance!!
Robert Hartley, Centre for Cell Engineering,University of Glasgow,
Glasgow G12 8QQ Tel:++44(0)141 330 4756
Fax:++44 (0)141 330 3730 mailto:rh at
Web :

More information about the Methods mailing list