In article <3A61D0C9.5C3442D0 at weizmann.ac.il>, yin.wang at weizmann.ac.il wrote:
For a strategy plan have a look at Gene Cloning by RT-PCR. Siebert and
Larick, specifically the chapter by Steve Winder.
This will show a comparison of vector/method etc (not specific to your
vector but worth a read.
Bob; VERY Sunny Scotland
> Hi all,
> I'm looking for an efficient way to clone 1.6 and 2 kb PCR products into
> pET 20b(+) vector,after trying in vain with cohesive end[EcoR1 ,Hind111]
> ligation for months.
> Thanks in advance!!
Robert Hartley, Centre for Cell Engineering,University of Glasgow,
Glasgow G12 8QQ Tel:++44(0)141 330 4756
Fax:++44 (0)141 330 3730 mailto:rh at mblab.gla.ac.uk
Web : http://www.gla.ac.uk/Inter/CellEngineering