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cloning PCR products

P.J. Shaw pjs14 at le.ac.uk
Mon Jan 15 04:52:18 EST 2001

On Sun, 14 Jan 2001, Yin Wang wrote:

> Hi all,
> I'm looking for an efficient way to clone 1.6 and 2 kb PCR products into
> pET 20b(+) vector,after trying in vain with cohesive end[EcoR1 ,Hind111]
> ligation for months.
> Thanks in advance!!
Try TOPO cloning in pCRII (Invitrogen). It is foolproof and definitely
worth the money in terms of time and effort saved. From that, clone into
your desired pET vector. Should get buckets of fragment with the reuired
sticky ends.

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