cloning PCR products

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Jan 15 04:17:56 EST 2001


In article <3A61D0C9.5C3442D0 at weizmann.ac.il>, the eminent Yin Wang at
The Hebrew University of Jerusalem wrote
>I'm looking for an efficient way to clone 1.6 and 2 kb PCR products into
>pET 20b(+) vector,after trying in vain with cohesive end[EcoR1 ,Hind111]
>ligation for months.
>Thanks in advance!!

Two step?

TA vector, confirm it is there, sequence, then cut out fragment and
clone as normal into PET20 etc.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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