cloning PCR products

Dr. Duncan Clark Duncan at
Mon Jan 15 04:17:56 EST 2001

In article <3A61D0C9.5C3442D0 at>, the eminent Yin Wang at
The Hebrew University of Jerusalem wrote
>I'm looking for an efficient way to clone 1.6 and 2 kb PCR products into
>pET 20b(+) vector,after trying in vain with cohesive end[EcoR1 ,Hind111]
>ligation for months.
>Thanks in advance!!

Two step?

TA vector, confirm it is there, sequence, then cut out fragment and
clone as normal into PET20 etc.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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