sucrose ulilization by e. coli

Sergio sergioal at bbm1.ucm.es
Mon Jan 15 09:48:01 EST 2001


as far as I know DH5a can use sucrose. Usually i add 0,2-0,4 % p/v on
M63 or M9.
i don't know about JM109...

hope this helps

Sergio

T V Naga Rama Chander wrote:

> hello,
> do these commercially available E. coli cells: JM109, DH5alpha etc.
> ulilize sucrose as carbon source?
> any reference information/tips are welcome.
> thanks in advance.
> plant tissue culture division        !
> national chemical laboratory         !
> pune-411008.                         !
> india.                               !
> tel: 91-20-5893300 ext: 2219         !
> fax: 91-20-5893438                   !
> e-mail: chander at kelvin.ncl.res.in    !
>
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