IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

crosslinking reagents for nuclear protein complexes

Britney I'mNotReallyBritneySoPleaseDon'tEmailMe at hotmail.com
Tue Jan 16 00:53:29 EST 2001


Dear All,
I am interested in analysing the protein constituents of a large protein
complex within the nucleas. I want to try taking a nuclear extract,
adding a crosslinker, immunoaffinity purifying the complex, reducing the
crosslinker, running the proteins on 1D- and/or 2D PAGE gels, then use
Mass spectrometry to identify the proteins. There are two protein
crosslinkers I want to purchase: DSP (Lomants reagent) and BASED. Before
I make the purchase, can anyone offer any comments or suggestions on
these or alternative crosslinkers?

Thanks,
Lyndal
(Sydney)






More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net