Annealing palindromic linkers

Bruno Cenni bruno.removethis.cenni at removethis.insel.ch
Tue Jan 16 02:43:25 EST 2001


Hi all,

we're (expectedly) having troubles cloning a linker into a plasmid. This
linker consists of two complementary 33 bp oligos that after annealing have
open restriction enzyme sites at their ends. The problems comes probably
from the fact that the oligos contain two complementary inverted 6 bp
recognition sites thus giving a palindrome (spaced by 3 bp). Any suggestions
on conditions to increase the proper annealing of the oligos without too
much of the self-annealing and stem-loop formation?

TIA

Bruno







More information about the Methods mailing list