RNA recovery from formamide

tfitzwater at gilead.com tfitzwater at gilead.com
Tue Jan 16 12:24:23 EST 2001


>Krzysztof Wasowicz (wasowicz at moskit.uwm.edu.pl)
>Mon 15 Jan 2001 - 12:48:46 GMT


>Hi,
>Maybe someone can write me how to recover RNA dissolved in formamide.
>It is recommended for storage, but I could not find the way to get it
>back to water solution. Or, maybe it is possible to use solution in
>formamide directly for RT-PCR and other applications. Thanks in
>advance for your replies.
>Please, e-mail me.


>Krzysztof Wasowicz, DVM, Ph.D.
>Dept of Animal Anatomy
>University of Agriculture and Technology
>Bldg 105J
>10-957 Olsztyn-Kortowo II
>Poland
>Phone:+48-89-5233733,5233688, Fax:+48-89-5234986
>e-mail:wasowicz at moskit.art.olsztyn.pl

I  have  seen  a  report  that  to remove formamide from RNA, you can add 4
volumes of ethanol and precipitate.

Tim Fitzwater
Principal Research Associate
Gilead Sciences


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