Blunting sheared DNA

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue Jan 16 20:35:54 EST 2001


At 09:43 AM 1/15/01 GMT, Chris Boyd wrote:
>
>"Dr. Hiranya S. Roychowdhury" <hroychow at nmsu.edu> wrote:
>: Never done with sheared DNA, but I would think that T4 DNA polymerse would
>: work with its 3-5' exo and 5-3' poly activity.
>
>... remembering to add an excess of dNTPs with the polymerase.
>
>Best wishes,
>-- 
>Chris Boyd                      | from, but \    Medical Genetics Section
>Chris.Boyd at ed.ac.uk             | not for,  /    MMC, Edinburgh Uni.,
>http://www.ed.ac.uk/~cboyd                       EH4 2XU, SCOTLAND
>NEW! transgenics / CF gene therapy job:
http://jobs.ac.uk/jobfiles/HH286.html
>
>


Indeed ... most certainly :-)
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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