WTD: Prep for BAC sequencing

P. Rocha pscfr2 at hermes.cam.ac.uk
Wed Jan 17 04:20:45 EST 2001


Hello,

I would be grateful if someone could make available a protocol for BAC DNA
preparation for sequencing. For my purposes I would prefer mini/midi
preps, but protocols for large preps are equally welcomed.

[Any tips on how to get best results with an ABI377 machine would also be
helpful].

Thanks in advance,

Pedro Rocha

-----------------------------
Dept. of Genetics
U. of Cambridge

E-mail: pscfr2 at cam.ac.uk


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