Does anyone know how to obtain the best separation of small
oligonucleotides on acrylamide. I need to separate oligonucleotides in
the range of 20 to 40 mers. In the moment I am running a 36 cm long 6%
arylamide gel (1:19 acryl:bisacrylamide) with 8 M Urea in 1XTBE (ABI377,
Applied Biosystems). Is there a more optimal acryl:bisacrylamide ratio
for sep of small oligoes. Has anyone got any experience on that?
PhD student Mette Nyegaard
KKA, Odense University Hospital