I had attempted to clone a 5kb insert (from a genomic library) into
the bluescript vector atleast 3 times (into Kpn I - Sma I sites) with
nosuccess. I am doing this only to sequence my inserts. I do a blue
white screening, none of my white colonies contain the insert. One
of my colleagues suggested using the pUC 19 vector.
Any suggestions ??
You can email me at
vasant at plantpath.ksu.edu
Thanks in advance