Cloning 5kb inserts

Janakiraman Vasantharajan vasant at PLANTPATH.KSU.EDU
Wed Jan 17 09:56:15 EST 2001


Hi netters,
I had attempted to clone a 5kb insert (from a genomic library) into 
the bluescript vector atleast 3 times (into Kpn I - Sma I sites) with 
nosuccess. I am doing this only to sequence my inserts.  I do a blue 
white screening, none of my white colonies contain the insert. One 
of my colleagues suggested using the pUC 19 vector. 
Any suggestions ?? 
You can email me at 
vasant at plantpath.ksu.edu
Thanks in advance  


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